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Staff Scientist

Dragan Maric, Ph.D.

Flow and Imaging Cytometry Core Facility

Facility Manager
Building 10 Room 5N-240A (office) 5S-241 (lab)
Center Drive MSC1444
Bethesda MD 20892-3704
Office: (301) 402-1406
Lab: (301) 402-1406

maricd@ninds.nih.gov

Dr. Dragan Maric received B.S. degrees in biochemistry and microbiology in 1981 from University of Maryland, and a M.S. in immunology in 1985 and a Ph.D. in neuroimmunology in 1989 from University of Belgrade, Yugoslavia. Dr. Maric completed his postdoctoral training in developmental neurobiology at NINDS from 1991-1993 and in mucosal immunology at McMaster University, Hamilton, Canada from 1993-1994. After returning to NINDS in 1994, Dr. Maric worked in the Laboratory of Neurophysiology first as a visiting scientist from 1994-2000 and then as a staff scientist from 2000-2010. He became Manager of the NINDS Flow and Imaging Cytometry Core Facility in 2001.



Dr. Maric’s primary interests include: researching the changing seminal properties of neural stem cells (NSCs) and their differentiating progeny during embryonic, postnatal and adult CNS development and adapting new methodologies to more effectively study the complex systems biology in CNS tissues under physiological and pathophysiological conditions. Dr. Maric is continuously developing novel multiplex fluorescence flow cytometry and in situ cytometry imaging bioassays aimed toward elucidating the underlying molecular and cellular mechanisms of NSC fate determination and regenerative potential during normal CNS development and in specific neuropathological and neurodegenerative disorders. These studies also involve the longitudinal assessment of in vivo survival, differentiation and integration of FACS-sorted embryonic NSCs after implantation of these cells into the rodent brains following ischemic and traumatic brain injuries. He is also developing state-of-the-art multiplex fluorescence immunohistochemistry biomarker screening methods utilizing unique combinations up to 100 biomarkers relevant to comprehensively study the complex processes brain tissue remodeling and recovery in response to different types of insults (including rodent models of stroke as illustrated in the image below) and other neurodegenerative disorders in the CNS.

DraganMaricResearchLab.jpgThe image shows complex tissue remodeling in the coronal section of a rat brain following focal ischemic brain injury. The section was simultaneously probed with 12 different fluorescent biomarkers relevant to neuroinflammation, neuronal plasticity, neurogenesis, gliogenesis and angiogenesis and each biomarker imaged in a separate fluorescence channel using multi-spectral wide field fluorescence microscopy. The images were then merged into a 12-color fluorescence composite to illustrate the concurrent processes of brain tissue damage and repair in response to focal ischemia. The above methodology exemplifies a comprehensive multiplex biomarker screening approach to study complex systems biology in the brain under normal and pathological conditions assayed at single cell level of spatial resolution.

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  • Andrea Sedlock, M.S.
    Biologist
    301-402-6934

  • 1) Bogoslovsky T, Bernstock JD, Bull G, Gouty S, Cox BM, Hallenbeck JM, Maric D (2018)
  • Development of a systems-based in situ multiplex biomarker screening approach for the assessment of immunopathology and neural tissue plasticity in male rats after traumatic brain injury
  • J Neurosci Res , 2018 Apr;96(4), 487-500. doi: 10.1002/jnr.24054. PMID: 28463430
  • 2) Pothayee N, Maric D, Sharer K, Tao-Cheng JH, Calac A, Bouraoud N, Pickel J, Dodd S, Koretsky A (2018)
  • Neural precursor cells form integrated brain-like tissue when implanted into rat cerebrospinal fluid
  • Commun Biol , Aug 14;1:114, doi: 10.1038/s42003-018-0113-8. PMID:30271994
  • 3) Bernstock JD, Peruzzotti-Jametti L, Ye D, Gessler FA, Maric D, Vicario N, Lee YJ, Pluchino S, Hallenbeck JM (2017)
  • Neural stem cell transplantation in ischemic stroke: A role for preconditioning and cellular engineering
  • J Cereb Blood Flow Metab , 37, 2314-2319. PMID: 28303738
  • 4) Maric D, Fiorio Pla A, Chang YH, Barker JL (2007)
  • Self-renewing and differentiating properties of cortical neural stem cells are selectively regulated by basic fibroblast growth factor (bFGF) signaling via specific FGF receptors
  • J Neurosci , 27, 1836-1852
  • 5) Maric D, Barker JL (2005)
  • Fluorescence-based sorting of neural stem cells and progenitors
  • Current Protocols in Neuroscience, 33 (Unit 3.18), 1-30
  • 6) Fiorio Pla A, Maric D, Brazer SC, Giacobini P, Liu X, Chang YH, Ambudkar IS, Barker JL (2005)
  • Canonical transient receptor potential 1 plays a role in basic fibroblast growth factor (bFGF)/FGF receptor-1-induced Ca2+ entry and embryonic rat neural stem cell proliferation
  • J Neurosci , 25, 2687-2701
  • 7) Maric D, Barker JL (2004)
  • Neural stem cells redefined: a FACS perspective
  • Molec Neurobiol, 30, 49-76
  • 8) Maric D, Maric I, Chang YH, Barker JL (2003)
  • Prospective cell sorting of embryonic rat neural stem cells and neuronal and glial progenitors reveals selective effects of basic fibroblast growth factor and epidermal growth factor on self-renewal and differentiation
  • J Neurosci , 23, 240-251
  • 9) Maric D, Liu QY, Maric I, Chaudry S, Chang YH, Smith SV, Sieghart W, Fritschy JM, Barker JL (2001)
  • GABA expression dominates neuronal lineage progression in the embryonic rat neocortex and facilitates neurite outgrowth via GABAA autoreceptor/Cl- channels
  • J Neurosci , 21, 2343-2360
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