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NINDSNIMHNICHDNIDCDNEINIDCRNIANIAAANIDANHGRI NCCIHNIDDKNIEHSCCB

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Investigator

Guohong Cui, M.D., Ph.D.

Neurobiology Laboratory / In Vivo Neurobiology Group


F175 Rall Building 111 T W Alexander Dr
Research Triangle Park NC 27709
Office: 984-287-3446
Lab: 984-287-3446
Fax: 301-480-3483
cuig@mail.nih.gov

Cui received his M.D. in clinical medicine from Beijing Medical University, now Peking University Health Science Center, in 1998 and a Ph.D. in neuroscience from The University of Texas at Austin in 2007. His doctoral work with Hitoshi Morikawa, M.D., Ph.D., focused on calcium signaling in midbrain dopamine neurons in brain slices. He joined the lab of Rui Costa, D.V.M., Ph.D., at the National Institute on Alcohol Abuse and Alcoholism (NIAAA) to expand his research to a systems level. Later, he joined the lab of David Lovinger, Ph.D., also at NIAAA. He came to NIEHS as a tenure-track investigator in 2014.



Guohong Cui, M.D., Ph.D., leads the In Vivo Neurobiology Group and holds a secondary appointment in the NIEHS Reproductive and Developmental Biology Laboratory. The In Vivo Neurobiology Group aims to synthesize task-specific neural activity maps in the normal brain and in animal models of neurological disorders.

Voluntary movement, as simple as pressing a lever to obtain a food reward, requires coordinated activation of multiple brain circuits responsible for processing motivation, movement planning and movement execution. Malfunction in any of these circuits may cause severe psychiatric and neurological conditions. The In Vivo Neurobiology Group studies this ‘simple’ behavior by monitoring and manipulating the molecular and cellular events in genetically defined groups of neurons in behaving animals.

Cui and his group have developed an in vivo fiber-optics based photometry method that can measure the fluorescence signals in deep brain structures in freely moving animals. By combining this method with genetically encoded fluorescent sensors, they were able to show that the two major projection pathways in the basal ganglia, which had been thought to have opposing effects on voluntary movement, were both activated during action initiation. The findings suggest that a well-organized co-activation of both pathways is required for initiating specific movements.

To obtain more detailed local and whole brain activity patterns, the In Vivo Neurobiology Group is developing new in vivo optical methods in two directions. First, to simultaneously measure the neural activity in discrete brain regions, the group is developing a multi-channel optical fiber array system that can monitor up to 8 regions of interest bilaterally in freely moving animals. Second, to achieve cellular resolution in a localized brain region, group members are building GRIN lens based deep brain imaging systems in both head-restrained and freely moving animals.

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  • 1) Juhee Haam, Jingheng Zhou, Guohong Cui and Jerrel L. Yakel (2018)
  • Septal cholinergic neurons gate hippocampal output to entorhinal cortex via oriens lacunosum moleculare interneurons
  • PNAS 2018
  • 2) Kupferschmidt DA, Juczewski K, Cui G, Johnson KA, Lovinger DM (2017)
  • Parallel, but Dissociable, Processing in Discrete Corticostriatal Inputs Encodes Skill Learning
  • Neuron, 96(2), 476-489
  • 3) Cui G, Jun SB, Jin X, Luo G, Pham MD, Lovinger DM, Vogel SS, Costa RM (2014)
  • Deep brain optical measurements of cell type-specific neural activity in behaving mice
  • Nat Protoc, 9(6), 1213-1228
  • 4) Sgobio C, Kupferschmidt DA, Cui G, Sun L, Li Z, Cai H, Lovinger DM (2014)
  • Optogenetic measurement of presynaptic calcium transients using conditional genetically encoded calcium indicator expression in dopaminergic neurons
  • PLoS One, 9(10), e111749
  • 5) Cui G, Jun SB, Jin X, Pham MD, Vogel SS, Lovinger DM, Costa RM (2013)
  • Concurrent activation of striatal direct and indirect pathways during action initiation
  • Nature, 494(7436), 238-242
  • 6) Cui G, Bernier BE, Harnett MT, Morikawa H (2007)
  • Differential regulation of action potential- and metabotropic glutamate receptor-induced Ca2+ signals by inositol 1,4,5-trisphosphate in dopaminergic neurons
  • J Neurosci, 27(17), 4776-4785
  • 7) Cui G, Okamoto T, Morikawa H (2004)
  • Spontaneous opening of T-type Ca2+ channels contributes to the irregular firing of dopamine neurons in neonatal rats
  • J Neurosci , 24(49), 11079-11087
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